https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51619 Wed 28 Feb 2024 16:01:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52054 Wed 27 Sep 2023 15:30:47 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52027 Wed 27 Sep 2023 09:54:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44865 Wed 26 Oct 2022 17:23:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45287 Wed 26 Oct 2022 17:20:21 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45061 NCPI > 1). However, 91.97% of the crop samples were uncontaminated according to the Chinese Risk Control Standard. Soils from the vegetable patch showed higher pollution level comparison with that from grain field and orchard. Health risks for both non-carcinogenic and carcinogenic risks were calculated through crop ingestion exposure pathway. Grain samples showed highest health risks, followed by melon and fruit, and vegetables. The health risks of crops were mainly driven by Cr and Cd. Crop consumption may pose risks for children but not adults. The source of trace element contamination in the different farmland soils varied and may be attributed to the different agricultural activities. Plant type had a greater influence on the trace element accumulation in crops compared with soil trace element contents and physicochemical properties.]]> Wed 26 Oct 2022 16:07:43 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45278 Wed 26 Oct 2022 16:06:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45164 Wed 26 Oct 2022 14:11:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45093 Wed 26 Oct 2022 12:23:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45080 Wed 26 Oct 2022 11:55:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44996 Batrachochytrium dendrobatidis is an emerging infectious pathogen present on every continent except Antarctica. It causes the disease chytridiomycosis in a subset of species but does not always result in disease or death for every host. Ambient temperature influences both amphibian metabolism and chytrid pathogenicity, however the interactive effects on host physiology is not well understood. We investigated the sublethal effect of B. dendrobatidis infection on a susceptible host, Litoria aurea to test (1) whether the infection load, metabolic activity, body fat and gonad size differed in L. aurea at either 24 °C or 12 °C ambient temperatures and (2) whether previous Bd infection caused long-term changes to body fat and gonad size. Litoria aurea in 12 °C treatments had higher infection loads of B. dendrobatidis and lower survivorship. Metabolic rate was higher and fat mass was lower in infected individuals and in animals in 24 °C treatments. Male L. aurea previously infected with B. dendrobatidis had smaller testes 5 months-post clearance of infection, an effect likely to translate to fitness costs in wild populations. These experiments demonstrate a physiological cost to sublethal B. dendrobatidis infection, which suggests a reduction in host fitness mediated by temperature in the host’s environment regardless of whether infection leads to mortality.]]> Wed 26 Oct 2022 09:28:56 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31071 CLU−/−) mice persisted after bleomycin and it was associated with increased DNA damage, reduced DNA repair responses, and elevated cellular senescence. Remarkably, this pattern mirrored that observed in IPF lung tissues. Together, our results show that cellular localization of Clusterin leads to divergent effects on epithelial cell regeneration and lung repair during fibrosis.]]> Wed 24 Nov 2021 15:51:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50683 Wed 24 Apr 2024 11:33:08 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52379 Wed 24 Apr 2024 09:45:59 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39164 Wed 22 Mar 2023 18:13:39 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41962 Wed 22 Mar 2023 18:11:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36666 Wed 22 Jul 2020 16:15:40 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54897 Wed 20 Mar 2024 13:32:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54883 Wed 20 Mar 2024 13:20:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44651 Wed 19 Oct 2022 09:56:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49890 Wed 14 Jun 2023 17:16:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52460 Wed 11 Oct 2023 15:02:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29783 dif. In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out two pairs of sequential strand exchange reactions. However, what makes the reaction unique among site-specific recombination reactions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-terminus of the FtsK DNA translocase. FtsK is a powerful molecular motor that functions in cell division, co-ordinating division with clearing chromosomal DNA from the site of septation and also acts to position the dif sites for recombination. This is a model system for unlinking, separating and segregating large DNA molecules. Here we describe the molecular detail of the interaction between XerD and FtsK that leads to activation of recombination as deduced from a co-crystal structure, biochemical and in vivo experiments. FtsKγ interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data.]]> Wed 11 Apr 2018 17:12:14 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22104 in vitro measures of fibrosis: cell attachment, proliferation and viability, and ECM deposition, were examined. Among these peptides, peptide 1C1 (FBLN1C1) enhanced ASM cell and fibroblast attachment. FBLN1C1 increased mitochondrial activity and proliferation in fibroblasts. In addition, FBLN1C1 stimulated fibulin1 deposition in PF and COPD fibroblasts, and augmented fibronectin and perlecan deposition in all three groups. Peptides FBLN1C2 to FBLN1C7 had no activity. The active fibulin-1C peptide identified in this study describes a useful tool for future studies. Ongoing investigation of the role of fibulin-1 may reveal the mechanisms underlying the pathphysiology of chronic lung diseases.]]> Wed 11 Apr 2018 16:41:15 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30223 In vitro antivenom efficacy studies were compared to rodent lethality studies to test two Indian snake antivenoms (VINS and BHARAT) against four Sri Lankan snakes. In vitro efficacy was tested at venom concentrations consistent with human envenoming. Efficacy was compared statistically for one batch from each manufacturer where multiple vials were available. In binding studies EC₅₀ for all VINS antivenoms were less than BHARAT for D. russelii [553 μg/mL vs. 1371 μg/mL;p=0.016), but were greater for VINS antivenoms compared to BHARAT for N. naja [336 μg/mL vs. 70 μg/mL;p<0.0001]. EC₅₀ of both antivenoms was only slighty different for E. carinatus and B. caeruleus. For procoagulant activity neutralisation, the EC₅₀ was lower for VINS compared to BHARAT - 60 µg/mL vs. 176 µg/mL (p<0.0001) for Russell's viper and 357 µg/mL vs. 6906µg/mL (p<0.0001) for Saw-scaled viper. Only VINS antivenom neutralized in vitro neurotoxicity of krait venom. Both antivenoms partially neutralized cobra and didn't neutralize Russell's viper neurotoxicity. Lethality studies found no statistically significant difference in ED₅₀ values between VINS and BHARAT antivenoms. VINS antivenoms appeared superior to BHARAT at concentrations equivalent to administering 10 vials antivenom, based on binding and neutralisation studies. Lethality studies were inconsistent suggesting rodent death may not measure relevant efficacy outcomes in humans.]]> Wed 11 Apr 2018 16:22:51 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14867 Wed 11 Apr 2018 15:51:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26195 Wed 11 Apr 2018 15:00:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27311 Wed 11 Apr 2018 14:08:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29296 DEAD (Asp-Glu-Ala-Asp) box protein 4 (Ddx4) gene promoter. Histopathological and functional analysis of ovaries from these mutant mice (Ctnnb1^ex3cko) showed no defects in ovarian functions, oocytes, ovulation and early embryonic development. However, breeding of the Ctnnb1^ex3cko female mice with males of known fertility never resulted in birth of mutant pups. Examination of uteri from time pregnant mutant females revealed defects in ectoderm differentiation leading to abnormal foetal development and premature death. Collectively, our work has established the role of active WNT/βcatenin signalling in oocyte biology and foetal development, and provides novel insights into the possible mechanisms of complications in human pregnancy such as repeated spontaneous abortion, sudden intrauterine unexpected foetal death syndrome and stillbirth.]]> Wed 11 Apr 2018 13:03:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30807 Wed 11 Apr 2018 11:36:06 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30612 18F-Florbetaben, was employed to categorise participants as low NAL (standard uptake value ratio; SUVR < 1.35, N = 65) and high NAL or preclinical AD (SUVR ≥ 1.35, N = 35) wherein, linear models were employed to compare FA compositions between the two groups. Increased arachidonic acid (AA, p < 0.05) and decreased docosapentaenoic acid (DPA, p < 0.05) were observed in high NAL. To differentiate low from high NAL, the area under the curve (AUC) generated from a ‘base model’ comprising age, gender, APOEε4 and education (AUC = 0.794) was outperformed by base model + AA:DPA (AUC = 0.836). Our findings suggest that specific alterations in erythrocyte FA composition occur very early in the disease pathogenic trajectory, prior to cognitive impairment. As erythrocyte FA levels are reflective of tissue FA, these alterations may provide insight into the pathogenic mechanism(s) of the disease and may highlight potential early diagnostic markers and therapeutic targets.]]> Wed 11 Apr 2018 11:27:55 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17004 Wed 11 Apr 2018 10:48:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30938 Wed 11 Apr 2018 10:38:07 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22757 Wed 11 Apr 2018 09:58:00 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30805 Wed 11 Apr 2018 09:19:15 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24837 Wed 10 Nov 2021 15:13:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35830 Wed 09 Mar 2022 16:02:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36837 Wed 08 Jul 2020 16:15:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44010 Wed 05 Oct 2022 15:12:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52196 Wed 04 Oct 2023 15:07:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38369 Wed 01 Sep 2021 11:09:32 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52724 Tue 24 Oct 2023 08:38:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38292 Tue 24 Aug 2021 12:58:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36665 max = 91 ± 1%) in pre-contracted mesenteric arteries. In contrast, E. ocellatus venom (1 μg/ml) only produced a maximum relaxant effect of 27 ± 14%, suggesting that rapid cardiovascular collapse is unlikely to be due to peripheral vasodilation. The prevention of rapid cardiovascular collapse, by 'priming' doses of venom, supports a role for depletable endogenous mediators in this phenomenon.]]> Tue 23 Jun 2020 12:29:07 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53302  0.9). We observed the strongest evidence to date for OPRM1: lead SNP rs9478500 (p = 2.56 × 10–9). Gene-based analyses identified novel genome-wide significant associations with PPP6C and FURIN. Variants within these loci appear to be pleiotropic for addiction and related traits.]]> Tue 21 Nov 2023 12:03:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48564 Tue 21 Mar 2023 15:51:48 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38712 Tue 18 Jan 2022 12:59:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35327 Tue 16 Jul 2019 11:58:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37522 230Th content. This poses problems in establishing reliable trends and periodicities for droughts and pluvial episodes in a region vulnerable to climate change. Here we constrain the chronology of a Cook Islands stalagmite using synchrotron µXRF two-dimensional mapping of Sr concentrations coupled with growth laminae optical imaging constrained by in situ monitoring. Unidimensional LA-ICP-MS-generated Mg, Sr, Ba and Na variability series were anchored to the 2D Sr and optical maps. The annual hydrological significance of Mg, Sr, Ba and Na was tested by principal component analysis, which revealed that Mg and Na are related to dry-season, wind-transported marine aerosols, similar to the host-rock derived Sr and Ba signatures. Trace element annual banding was then used to generate a calendar-year master chronology with a dating uncertainty maximum of ± 15 years over 336 years. Our approach demonstrates that accurate chronologies and coupled hydroclimate proxies can be obtained from speleothems formed in tropical settings where low seasonality and problematic U–Th dating would discourage the use of high-resolution climate proxies datasets.]]> Tue 15 Nov 2022 14:35:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53025 Tue 14 Nov 2023 14:30:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35829 Tue 10 Dec 2019 15:45:23 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36575 Tue 09 Jun 2020 11:47:41 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38407 60 kilometre long, >10 metre thick valley-wide deposit of finely laminated mud. This previously unrecognised sediment trap persisted from 8,518 to 5,067 cal yr BP preventing sediment delivery to the marine environment. Its identification requires that mid-Holocene climate reconstructions for southeastern Australia based on fluctuations in the delivery of fine-grained sediment to the ocean offshore the lower Murray River's mouth must be re-evaluated.]]> Tue 07 Sep 2021 12:17:32 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39827 Tue 06 Jun 2023 09:44:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43873 Tue 04 Oct 2022 12:29:00 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30822 50 loci at which common variants with minor allele frequency >5% are associated with kidney function. GWAS using more complete reference sets for imputation, such as those from The 1000 Genomes project, promise to identify novel loci that have been missed by previous efforts. To investigate the value of such a more complete variant catalog, we conducted a GWAS meta-analysis of kidney function based on the estimated glomerular filtration rate (eGFR) in 110,517 European ancestry participants using 1000 Genomes imputed data. We identified 10 novel loci with p-value < 5 × 10−8 previously missed by HapMap-based GWAS. Six of these loci (HOXD8, ARL15, PIK3R1, EYA4, ASTN2, and EPB41L3) are tagged by common SNPs unique to the 1000 Genomes reference panel. Using pathway analysis, we identified 39 significant (FDR < 0.05) genes and 127 significantly (FDR < 0.05) enriched gene sets, which were missed by our previous analyses. Among those, the 10 identified novel genes are part of pathways of kidney development, carbohydrate metabolism, cardiac septum development and glucose metabolism. These results highlight the utility of re-imputing from denser reference panels, until whole-genome sequencing becomes feasible in large samples.]]> Tue 04 Apr 2023 19:09:51 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50654 Tue 01 Aug 2023 12:10:37 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51323 Thu 31 Aug 2023 14:28:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30656 Thu 28 Oct 2021 13:03:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45385 Thu 27 Oct 2022 16:12:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45124 Thu 27 Oct 2022 10:53:06 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44983 in vitro upregulated steroidogenic genes but did not fully induce LC differentiation. In vivo, no difference in LC-regeneration was noted between Sham and hAd-PSC-transplanted rats. Interestingly, Cyp17a1 expression increased in hAd-PSC-transplanted testes compared to intact vehicle controls and the luteinising hormone/testosterone ratio returned to Vehicle control levels which was not the case in EDS + Sham animals. Notably, hAd-PSCs were undetectable one-month after transplantation suggesting this effect is likely mediated via paracrine mechanisms during the initial stages of regeneration; either directly by interacting with regenerating LCs, or through indirect interactions with trophic macrophages.]]> Thu 27 Oct 2022 09:17:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34486 Thu 27 Jan 2022 15:56:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37622 NTR and the proneurotrophin co-receptor sortilin, remains unclear. In the present study, analysis of these proteins was conducted by immunohistochemistry and digital quantification in a series of 204 lung cancers of different histological subtypes versus 121 normal lung tissues. TrkA immunoreactivity was increased in squamous cell carcinoma compared with benign and other malignant lung cancer histological subtypes (p < 0.0001). NGF and proNGF were also increased in squamous cell carcinoma, as well as in adenocarcinoma (p < 0.0001). In contrast, p75^NTR was increased across all lung cancer histological subtypes compared to normal lung (p < 0.0001). Sortilin was higher in adenocarcinoma and small cell carcinoma (p < 0.0001). Nerves in the tumor microenvironment were negative for TrkA, NGF, proNGF, p75^NTR and sortilin. In conclusion, these data suggest a preferential therapeutic value of targeting the NGF-TrkA axis in squamous cell carcinomas of the lung.]]> Thu 24 Mar 2022 11:36:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53384 Thu 23 Nov 2023 12:55:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37530 3)₂ or as N captured in field-aged biochar at five increasing N doses to quinoa and perennial ryegrass in pots. Interestingly, the biochar-captured N was as plant available as the mineral nitrate, except for the highest dosage. Refuting our hypothesis, no significant amounts of N were extractable at the end of the study from the biochar–soil mixtures with repeated-extraction protocols. Thus, N captured by biochar may improve the N use efficiency in agriculture. Further research should evaluate the role of biochar particle size, root morphology, mycorrhization, and soil moisture (variations) for nitrate retrieval from biochar particles by plants because the captured biochar N was less available in the field as under present controlled conditions.]]> Thu 21 Oct 2021 12:51:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39162 Thu 19 May 2022 19:17:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30150 Thu 17 Feb 2022 09:30:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46046 Thu 10 Nov 2022 09:29:01 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33473 Thu 09 Dec 2021 11:01:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52259 Thu 05 Oct 2023 18:33:05 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29940 Thu 04 Nov 2021 10:39:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36749 Thu 02 Jul 2020 17:27:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42690 Thu 01 Sep 2022 09:06:31 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50593 Sun 30 Jul 2023 16:47:01 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24939 Sat 24 Mar 2018 07:14:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50787 Sat 05 Aug 2023 11:22:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38628 + T cells of relapsing–remitting (RR) MS patients compared to healthy controls and identified differentially methylated regions (DMRs) in HLA-DRB1 and RNF39. This study aimed to investigate the DNA methylation profiles of the CD4⁺ T cells of progressive MS patients. DNA methylation was measured in two separate case/control cohorts using the Illumina 450K/EPIC arrays and data was analysed with the Chip Analysis Methylation Pipeline (ChAMP). Single nucleotide polymorphisms (SNPs) were assessed using the Illumina Human OmniExpress24 arrays and analysed using PLINK. Expression was assessed using the Illumina HT12 array and analysed in R using a combination of Limma and Illuminaio. We identified three DMRs at HTR2A, SLC17A9 and HDAC4 that were consistent across both cohorts. The DMR at HTR2A is located within the bounds of a haplotype block; however, the DMR remained significant after accounting for SNPs in the region. No expression changes were detected in any DMRs. HTR2A is differentially methylated in progressive MS independent of genotype. This differential methylation is not evident in RRMS, making it a potential biomarker of progressive disease.]]> Mon 29 Jan 2024 17:52:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53289 Mon 27 Nov 2023 15:51:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35542 Mon 26 Aug 2019 15:28:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51981 Mon 25 Sep 2023 12:30:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31990 Mon 23 Sep 2019 12:33:31 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42362 Mon 22 Aug 2022 14:08:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26596 Mon 20 Nov 2023 14:18:28 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43435 P_app), with differences in ML retention and complexation amongst the chelants and the gut microbes. The decrease in ML permeability varied amongst the MLs. Chelating agents reduce intestinal absorption of MLs by forming complexes thereby making them less permeable. In the case of gut bacteria, the decrease in the intestinal permeability of MLs may be associated to a direct protection of the intestinal barrier against the MLs or indirect intestinal ML sequestration by the gut bacteria through adsorption on bacterial surface. Thus, both gut microbes and chelating agents can be used to decrease the intestinal permeability of MLs, thereby mitigating their toxicity.]]> Mon 19 Sep 2022 11:35:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40790 Mon 18 Jul 2022 16:21:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40715 Mon 18 Jul 2022 11:27:29 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37249 Mon 14 Sep 2020 14:34:20 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38826 Mon 14 Feb 2022 15:24:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54758 Mon 11 Mar 2024 15:01:25 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54753 Mon 11 Mar 2024 14:53:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38182 Mon 09 Aug 2021 15:09:47 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45413 Fri 28 Oct 2022 12:09:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35689 Fri 25 Oct 2019 11:42:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30802 Fri 24 Aug 2018 08:58:46 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52660 Fri 20 Oct 2023 09:10:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53050 Fri 17 Nov 2023 12:07:55 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53194 Fri 17 Nov 2023 11:51:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55287 Fri 10 May 2024 16:22:11 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38322 50 value of 100 nM is greater than 500-fold more sensitive to NAP-6 compared with other tumour derived cell models. Within 1 h exposure of these cells to NAP-6, CYP1A1 expression increases 25-fold, rising to 250-fold by 24 h. A smaller concurrent increase in CYP1A2 and CYP1B1 is also observed. Within 24 h these cells present with DNA damage as evident by enhanced H2AXγ expression, cell cycle checkpoint activation via increased CHK2 expression, S-phase cell cycle arrest and cell death. Specific small molecule inhibitors of the AHR and CYP1 family ameliorate these events. A positive luciferase reporter assay for NAP-6 induced XRE binding further confirms the role of the AHR in this phenomenon. Non-sensitive cell lines fail to show these biological effects. For the first time we identify 2-(2-aminophenyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione as a new AHR ligand that selectively targets breast cancer.]]> Fri 03 Sep 2021 14:57:31 AEST ]]>